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KMID : 0350519950480030737
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Journal of Catholic Medical College
1995 Volume.48 No. 3 p.737 ~ p.744
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A Study of Balance between Oxidants and Antioxidants from the Peripheral Blood Neutrophils in Patients with Pulmonary Emphysema
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Abstract
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Cigarette smoking is firmly established as the primary environmental risk factor associated with pulmonary emphysema.
The mechanism by which smoking causes emphysema is unkonwn, but the activated lung phagocytes including pulmonary alveolar macrophages and neutrophils are suspected to be playing a major role in the development of emphysema. Phagocytes from
smokers
are
known to generate more oxidants than those from nonsmokers are very important in releasing oxidants and proteolytic lung damages.
In order to investigate the possible oxidant-antioxidant imbalance in peripheral blood neutrophils from the patients with pulmonary emphysema, the oxidant activity by measuring the level of superoxide and luminol enhanced chemiuminescence (LECL),
and
the antioxidant activity by measuring the ectivities of superoxide dismutase (SOD), catalase, glutathione peroxidase(GSH-Px) from the isolated peripheral blood neutrophils in patients with pulmonary emphysema were studied.
@ES The results were as follows ;
@EN 1. The amount of oxidant radicals which were reieased from activated neutrophils by PMA (phorbol myristate acetate) was not different between the control and the pulmonary emphysema groups when measured by superoxide (P>0.05) and
LECL(P>0.05).
2. The activities of Cu,Zn-SOD in the cytoplasm and mitochondria of neutrophils were not different between the control and the pulmonary emphysema groups (P>0.05).
3. The activities of catalase in the cytoplasm of neutrophils were more increased in the pulmonary emphysema than in the control groups(P<0.05), but no significant difference was noted in the mitochondria(P>0.05).
4. The activities of GSH-Px in the mitochondria of neutrophils were more increased in the pulmonary emphysema than in the control groups(P<0.05), but no significant difference was noted in the cytoplasm(P>0.05).'
These results suggest that the selective increase of antioxidant activity in neutrophils of pulmonary emphysema might be due to the defense mechanism against the increased oxidant burden by smoking in patients with pulmonary emphysema.
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